Fibrin and Serum as a Culture Medium
نویسنده
چکیده
A technique is described by which a medium composed of fibrinogen suspension, serum, and embryo juice may be made. Fibroblasts grew in this medium about as well as in plasma and embryo juice. A strain of connective tissue in this medium remained practically as active as the control for several passages.
منابع مشابه
P-83: Development of Mouse Preantral Follicles in Fibrin-Alginate Matrix during In Vitro Culture
Background This research was conducted to assess preantral follicle development in fibrin alginate matrix following ovarian tissue vitrification MaterialsAndMethods Ovaries of 13-day-old NMRI female mice were removed and placed in control and vitrification groups. Vitrification group ovaries were transferred in media containing ethylene glycol, dimethyl sulphoxide, and sucrose then were plunged...
متن کاملP-66: Optimization of Human Luteinizing Hormone Expression in CHO Cells Culture by Stepwise Reduction in Serum Concentration
Background: Mammalian Cell lines are the main expression system for the production of recombinant therapeutic proteins. Optimization of cell culture condition is performed via alteration in different parameter. Cell culture media plays an important role in cell cycle because of compounds such as amino acids, vitamins, inorganic salts, glucose, and serum as a source of growth factors, trace elem...
متن کاملComparison of the efficacy of Piascledine and transforming growth factor β1 on chondrogenic differentiation of human adipose-derived stem cells in fibrin and fibrin-alginate scaffolds
Objective(s):The aim of this study was to compare the chondrogenic induction potential of Piascledine and TGF-β1 on adipose-derived stem cells (ADSCs) in fibrin and fibrin-alginate scaffolds. Materials and Methods: Human subcutaneous adipose tissues were harvested from three patients who were scheduled to undergo liposuction. Isolated ADSCs were proliferated in a culture medium. Then, the cell...
متن کاملTechnique of Cultivating Human Tissues in Vitro
1. Unmodified human plasma is not a satisfactory culture medium for human tissues owing to the susceptibility of human fibrin to digestion by tissue ferments. The necessary framework is thus destroyed before the cells begin to migrate. The difficulty can be overcome by adding to human plasma or serum a small quantity of fowl or pigeon plasma, the fibrin of which is highly resistant to digestion...
متن کاملOptimization of Key Factors in Serum Free Medium for Production of Human Recombinant GM-CSF Using Response Surface Methodology
Researchers add serum to a classical medium at concentrations of 5 to 10% (v/v) to grow cellsin-vitro culture media. Unfortunately, serum is a poorly defined culture medium componentas its composition can vary considerably while serum-free cell culture media are an excellentalternative to standard serum-containing media and offer several major advantages. Advantagesof us...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of Experimental Medicine
دوره 33 شماره
صفحات -
تاریخ انتشار 2003